Chymopapain

Title: Chymopapain
CAS Registry Number: 9001-09-6
Manufacturers' Codes: BAX-1526
Trademarks: Chymodiactin (Smith); Discase (Boots)
Literature References: Proteolytic enzyme which is the major component of the crude latex of Carica papaya, Caricaceae. A sulfhydryl enzyme similar to papain, q.v., with respect to substrate specificities, but differing in electrophoretic mobility, stability and solubility. Original crystallization and partial characterization: Jansen, Balls, J. Biol. Chem. 137, 459 (1941); eidem, US 2313875 (1943). Consists of four components, two of which have molecular wts of about 35,000 and have been isolated and studied. Chymopapain A: Erbata, Yasunobu, J. Biol. Chem. 237, 1086 (1962); chymopapain B: Kunimitsu, Yasunobu, Biochim. Biophys. Acta 139, 405 (1966); Isunoda, Yasunobu, J. Biol. Chem. 241, 4610 (1966). Purification: Stern, US 3558433 (1971 to Baxter Labs.). Specificity studies: Ebata, Takahashi, Biochim. Biophys. Acta 118, 201 (1966). Reviews: Kunimitsu, Yasunobu, Methods Enzymol. 19, 244-252 (1970); Glazer, Smith, The Enzymes vol. III, P. D. Boyer, Ed. (Academic Press, New York, 3rd ed., 1971) pp 537-538. Review of use in herniated disk treatment: M. J. David, J. Am. Med. Assoc. 243, 2043 (1980).
Properties: Powder, more sol in aq soln than papain. Extremely stable at pHs as low as 1.8 and sol in satd NaCl above pH 3. Most active at pHs 2.5 to 4.0. uv max (pH 7): 280 nm (A1%1cm 18.7 (A); 18.4 (B)). The ratio of milk-clotting to proteolytic activity is twice that of papain although the clotting capacities are the same.
Absorption maximum: uv max (pH 7): 280 nm (A1%1cm 18.7 (A); 18.4 (B))
Use: In meat tenderizer.
Therap-Cat: Proteolytic enzyme (used in chemonucleolysis).
Keywords: Enzyme; Proteolytic.
Chymotrypsins Ciafos Cichoriin Ciclesonide Cicletanine

Chymopapain
Identifiers
EC number 3.4.22.6
CAS number 2593837
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Chymopapain
Clinical data
Legal status Prescription only
Routes Injection into intervertebral disc
Identifiers
ATC code M09AB01
UNII 1UK146T40N YesY
ChEMBL CHEMBL1201626 N
Chemical data
Formula  ?
 N (what is this?)  (verify)

Chymopapain (EC 3.4.22.6, chymopapain A, chymopapain B, chymopapain S, brand name Chymodiactin) is a proteolytic enzyme isolated from the latex of papaya (Carica papaya). It is a medication used to treat herniated lower lumbar discs in the spine.[1] Chymopapain injections are normally given under local, rather than general, anaesthesia. The dose for a single intervertebral disc is 2 to 4 nanokatals, with a maximum dose per patient of 8 nanokatals. The procedure is referred to as chemonucleolysis.

The sale and distribution of chymopapain was discontinued in the United States on January 27, 2003 after the company producing it decided to stop selling worldwide.[2][3]